![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 282, Issue 2, 1037-1043, 1997
Department of Pathology, We have previously shown that hepatic thromboxane production is
increased in experimental alcoholic liver disease. The present study
was designed to investigate the cell type in liver responsible for
increased thromboxane synthesis and the role of the thromboxane receptor system in the pathogenesis of liver injury. Male Wistar rats
were divided into four groups and fed a liquid diet with dextrose or
ethanol for 2, 4 and 8 weeks. Medium chain triglycerides or corn oil
provided the dietary fatty acids. Kupffer cells, endothelial cells and
hepatocytes were isolated from rats fed the different diets for 4 weeks. Liver histopathology, thromboxane synthase mRNA and protein,
thromboxane levels and thromboxane receptor mRNA were evaluated in each
group. In rats fed corn oil and ethanol, an increase in thromboxane
synthase and liver levels of thromboxane metabolites were significantly
higher than in the corn oil-dextrose-fed group and were correlated with
the presence of pathological changes in the liver. Kupffer cells showed
increased expression of thromboxane synthase. In rats fed medium chain
triglycerides and ethanol, the levels of thromboxane synthase mRNA and
protein were significantly lower than in the corn oil-ethanol-fed
groups (P < .01) and liver injury was absent. However, the levels
of thromboxane synthase mRNA, protein and thromboxane were
significantly higher in the medium chain triglyceride-ethanol-fed rats
than in the respective dextrose-fed controls. Among the different cell
types, thromboxane A2-receptor mRNA levels were highest in
the Kupffer cells in corn oil-ethanol-fed rats. The increase in
thromboxane synthase in Kupffer cells together with an increase in
thromboxane receptor levels suggests than thromboxanes may contribute
to liver injury in ethanol-fed rats.
Copyright © by The American Society for Pharmacology and Experimental Therapeutics
This article has been cited by other articles:
|
|
 |
|
  F. Mir and G. C. Le Breton A Novel Nuclear Signaling Pathway for Thromboxane A2 Receptors in Oligodendrocytes: Evidence for Signaling Compartmentalization during Differentiation Mol. Cell. Biol., October 15, 2008; 28(20): 6329 - 6341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, L. Kuo, and J. N. Stallone Estrogen potentiates constrictor prostanoid function in female rat aorta by upregulation of cyclooxygenase-2 and thromboxane pathway expression Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2444 - H2455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Tirapelli, J. Al-Khoury, G. Bkaily, P. D'Orleans-Juste, V. L. Lanchote, S. A. Uyemura, and A. M. de Oliveira Chronic Ethanol Consumption Enhances Phenylephrine-Induced Contraction in the Isolated Rat Aorta J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 233 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yokoyama, Y. Nimura, M. Nagino, K. I. Bland, and I. H. Chaudry Role of Thromboxane in Producing Hepatic Injury During Hepatic Stress Arch Surg, August 1, 2005; 140(8): 801 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yokoyama, B. Toth, W. C. Kitchens, M. G. Schwacha, K. I. Bland, and I. H. Chaudry Role of thromboxane in producing portal hypertension following trauma-hemorrhage Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1293 - G1299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gobeil Jr., S. G. Bernier, A. Vazquez-Tello, S. Brault, M. H. Beauchamp, C. Quiniou, A. M. Marrache, D. Checchin, F. Sennlaub, X. Hou, et al. Modulation of Pro-inflammatory Gene Expression by Nuclear Lysophosphatidic Acid Receptor Type-1 J. Biol. Chem., October 3, 2003; 278(40): 38875 - 38883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kono, M. Nakagami, I. Rusyn, H. D. Connor, B. Stefanovic, D. A. Brenner, R. P. Mason, G. E. Arteel, and R. G. Thurman Development of an animal model of chronic alcohol-induced pancreatitis in the rat Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1178 - G1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kono, I. Rusyn, T. Uesugi, S. Yamashina, H. D. Connor, A. Dikalova, R. P. Mason, and R. G. Thurman Diphenyleneiodonium sulfate, an NADPH oxidase inhibitor, prevents early alcohol-induced liver injury in the rat Am J Physiol Gastrointest Liver Physiol, May 1, 2001; 280(5): G1005 - G1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kono, I. Rusyn, B. U. Bradford, H. D. Connor, R. P. Mason, and R. G. Thurman Allopurinol Prevents Early Alcohol-Induced Liver Injury in Rats J. Pharmacol. Exp. Ther., April 1, 2000; 293(1): 296 - 303. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kono, M. D. Wheeler, I. Rusyn, M. Lin, V. Seabra, C. A. Rivera, B. U. Bradford, D. T. Forman, and R. G. Thurman Gender differences in early alcohol-induced liver injury: role of CD14, NF-kappa B, and TNF-alpha Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G652 - G661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kono, N. Enomoto, H. D. Connor, M. D. Wheeler, B. U. Bradford, C. A. Rivera, M. B. Kadiiska, R. P. Mason, and R. G. Thurman Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol Am J Physiol Gastrointest Liver Physiol, March 1, 2000; 278(3): G467 - G476. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
