Vol. 281, Issue 3, 1164-1170, 1997

Analgesia by Dihydrocodeine Is Not Due to Formation of Dihydromorphine: Evidence from Nociceptive Activity in Rat Thalamus

Ilmar Jurna, Wolfgang Kömen, Joseph Baldauf and Wolfgang Fleischer

Institut für Pharmakologie und Toxikologie der Universität des Saarlandes, D-66424 Homburg/Saar, Germany (I.J., W.K., J.B.), and Mundipharma GmbH, D-65549 Limburg/Lahn, Germany (W.F.)

Dihydrocodeine is increasingly used in slow-release preparations for the treatment of chronic pain on step 2 of the "analgesic ladder" of the World Health Organization. Dihydrocodeine is suggested to act after O-demethylation to dihydromorphine. To test this possibility, experiments were carried out on rats under urethane anesthesia in which nociceptive activity was evoked by electrical stimulation of afferent C fibers in the sural nerve and recorded from neurons in the ventrobasal complex of the thalamus. Dihydrocodeine administered by intravenous injection reduced the evoked nociceptive activity in a dose-dependent manner. Like morphine, dihydrocodeine was capable of completely suppressing the evoked activity. Maximum depression was caused by 2 mg/kg, and the ED₅₀ is 0.47 mg/kg. Naloxone (0.2 mg/kg) reversed the effect of dihydrocodeine (2 mg/kg). To inhibit O-demethylation of dihydrocodeine to dihydromorphine, metyrapone or cimetidine (50 mg/kg) was injected intraperitoneally 20 min before dihydrocodeine (1 and 2 mg/kg). This failed to markedly reduce the effect of dihydrocodeine. Dihydromorphine injected intravenously also reduced the evoked activity in a dose-dependent way. Maximum depression occurred at a dose of 4 mg/kg, and the ED₅₀ is 0.97 mg/kg. Dihydrocodeine and dihydromorphine were equieffective when administered by intrathecal injection at a dose of 100 µg. It is concluded that dihydrocodeine causes analgesia independent of biotransformation to dihydromorphine.