![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 281, Issue 2, 965-971, 1997
Department of Pharmacology, University of Colorado Health Sciences
Center Denver, Colorado (T.J.G., P.C.B.) and
Department of Veterans
Affairs Medical Center, Research Service, Denver, Colorado (P.C.B.)
Localization of age-related deficits in the cerebellar
beta adrenergic signal transduction cascade were
investigated electrophysiologically using forskolin (FORSK) and
adenosine-3
,5-cyclic monophosphothioate Sp-isomer (Sp-cAMPS) applied
via pressure ejection from extracellular multibarreled
glass electrodes to activate the transduction cascade. In young rats,
100 µM FORSK activated AC, and 100 µM Sp-cAMPS activated protein
kinase A; thus, both increased GABAergic inhibition of Purkinje cell
firing. In aged rats, however, 100 µM FORSK was unable to increase
GABAergic inhibition of Purkinje cell firing. In addition, 1 mM
7
-decacetyl-7-(
-N-methylpiperazino)butyryl-forskolin, an
analog of FORSK, was also unable to increase GABAergic inhibition in
aged rats. In contrast, Sp-cAMPS was able to increase GABAergic inhibition in aged rats, but higher doses were required than in young
rats. Isoproterenol (ISO), a beta adrenergic agonist,
was ineffective in increasing GABAergic inhibition of Purkinje firing in aged rats when tested alone, but ISO was effective in increasing Purkinje cell inhibition when ISO was tested with Sp-cAMPS. The results
of this experiment indicate that one age-related deficit in the
cerebellar beta adrenergic system occurs at the level of protein kinase A activation.
This article has been cited by other articles:
|
|
 |
|
  G. F. Reis, M. B. Lee, A. S. Huang, and K. D. Parfitt Adenylate Cyclase-Mediated Forms of Neuronal Plasticity in Hippocampal Area CA1 Are Reduced With Aging J Neurophysiol, June 1, 2005; 93(6): 3381 - 3389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A Joseph, B. Shukitt-Hale, and G. Casadesus Reversing the deleterious effects of aging on neuronal communication and behavior: beneficial properties of fruit polyphenolic compounds Am. J. Clinical Nutrition, January 1, 2005; 81(1): 313S - 316S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Cartford, A. Samec, M. Fister, and P. C. Bickford Cerebellar norepinephrine modulates learning of delay classical eyeblink conditioning: Evidence for post-synaptic signaling via PKA Learn. Mem., November 1, 2004; 11(6): 732 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Cartford, T. Gould, and P. C. Bickford A Central Role for Norepinephrine in the Modulation of Cerebellar Learning Tasks Behav Cogn Neurosci Rev, June 1, 2004; 3(2): 131 - 138. [Abstract] [PDF]
|
|
|
|
 |
|
 M. C. Cartford, C. Gemma, and P. C. Bickford Eighteen-Month-Old Fischer 344 Rats Fed a Spinach-Enriched Diet Show Improved Delay Classical Eyeblink Conditioning and Reduced Expression of Tumor Necrosis Factor alpha (TNFalpha ) and TNFbeta in the Cerebellum J. Neurosci., July 15, 2002; 22(14): 5813 - 5816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gemma, M. H. Mesches, B. Sepesi, K. Choo, D. B. Holmes, and P. C. Bickford Diets Enriched in Foods with High Antioxidant Activity Reverse Age-Induced Decreases in Cerebellar beta -Adrenergic Function and Increases in Proinflammatory Cytokines J. Neurosci., July 15, 2002; 22(14): 6114 - 6120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Clayton, M. H. Mesches, E. Alvarez, P. C. Bickford, and M. D. Browning A Hippocampal NR2B Deficit Can Mimic Age-Related Changes in Long-Term Potentiation and Spatial Learning in the Fischer 344 Rat J. Neurosci., May 1, 2002; 22(9): 3628 - 3637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Joseph, B. Shukitt-Hale, N. A. Denisova, D. Bielinski, A. Martin, J. J. McEwen, and P. C. Bickford Reversals of Age-Related Declines in Neuronal Signal Transduction, Cognitive, and Motor Behavioral Deficits with Blueberry, Spinach, or Strawberry Dietary Supplementation J. Neurosci., September 15, 1999; 19(18): 8114 - 8121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Joseph, B. Shukitt-Hale, N. A. Denisova, R. L. Prior, G. Cao, A. Martin, G. Taglialatela, and P. C. Bickford Long-Term Dietary Strawberry, Spinach, or Vitamin E Supplementation Retards the Onset of Age-Related Neuronal Signal-Transduction and Cognitive Behavioral Deficits J. Neurosci., October 1, 1998; 18(19): 8047 - 8055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Reis, M. B. Lee, A. S. Huang, and K. D. Parfitt Adenylate Cyclase-Mediated Forms of Neuronal Plasticity in Hippocampal Area CA1 Are Reduced With Aging J Neurophysiol, June 1, 2005; 93(6): 3381 - 3389. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
