![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 281, Issue 1, 448-453, 1997
Departments of
Medical Physiology (C.J.M., G.W.) and
Animal Science
(G.W.) Texas A&M University, College Station, Texas
This study was conducted to test the hypothesis that
L-glutamine has differential effects on nitric oxide (NO)
synthesis from L-arginine in bovine venular endothelial
cells (EC) stimulated by A23187 (a Ca++
ionophore) and receptor-mediated vasodilators (bradykinin and substance
P). EC were cultured at 37°C for 24 h in the presence of 0.4 mM
L-arginine and 0.0 to 2.0 mM L-glutamine with
or without 1 µM A23187, 1 µM bradykinin or 10 µM
substance P. The release of nitrite and nitrate by EC was used as an
indicator of NO synthesis. A23187, bradykinin or substance
P increased NO synthesis from L-arginine by EC in the
presence or absence of L-glutamine. The addition of
L-glutamine (0.5 and 2 mM) markedly increased intracellular concentrations of L-glutamine, L-glutamate and
L-aspartate and decreased NO synthesis by EC in a
concentration-dependent manner in the presence or absence of
A23187, bradykinin or substance P. L-Glutamine
had no effect on L-arginine uptake by EC or on intracellular L-arginine concentration. Neither
L-glutamine nor its glutaminase metabolites (ammonia,
L-glutamate and L-aspartate) had any effect on
endothelial NO synthase activity. Taken together, these results suggest
that the inhibition by L-glutamine of NO synthesis from
L-arginine is unlikely to result from an effect of
L-glutamine on L-arginine transport or NO
synthase activity. Although the mechanism involved remains unknown,
regulation of the arginine-NO pathway by L-glutamine may
have pharmacologic and therapeutic implications in such conditions as
inflammation and septic shock by inhibiting NO generation from
L-arginine in endothelial cells.
This article has been cited by other articles:
|
|
 |
|
  D. Schachter L-Glutamine in vitro regulates rat aortic glutamate content and modulates nitric oxide formation and contractility responses Am J Physiol Cell Physiol, July 1, 2007; 293(1): C142 - C151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kawaguchi, S. W. Brusilow, R. J. Traystman, and R. C. Koehler Glutamine-dependent inhibition of pial arteriolar dilation to acetylcholine with and without hyperammonemia in the rat Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1612 - R1619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kohli, C. J. Meininger, T. E. Haynes, W. Yan, J. T. Self, and G. Wu Dietary L-Arginine Supplementation Enhances Endothelial Nitric Oxide Synthesis in Streptozotocin-Induced Diabetic Rats J. Nutr., March 1, 2004; 134(3): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Mann, D. L. Yudilevich, and L. Sobrevia Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells Physiol Rev, January 1, 2003; 83(1): 183 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, C. J. Meininger, J. R. Hawker Jr., T. E. Haynes, D. Kepka-Lenhart, S. K. Mistry, S. M. Morris Jr., and G. Wu Regulatory role of arginase I and II in nitric oxide, polyamine, and proline syntheses in endothelial cells Am J Physiol Endocrinol Metab, January 1, 2001; 280(1): E75 - E82. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Okada, Y. Watanabe, S. W. Brusilow, R. J. Traystman, and R. C. Koehler Interaction of glutamine and arginine on cerebrovascular reactivity to hypercapnia Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1577 - H1584. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
