![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 281, Issue 1, 109-114, 1997
-glucuronide Antinociception1
The Cotzias Laboratory of Neuro-Oncology, Memorial Sloan-Kettering
Cancer Center, New York, New York (G.C.R., L.L., Y.-X.P., W.S.,
G.W.P.); Departments of
Neurology & Neuroscience and Pharmacology,
Cornell University Medical College, New York, New York (G.W.P.);
Neuropsychology Doctoral Sub Program, City University of New York, New
York, New York (L.L., J.C., R.J.B.); and
Department of Psychology,
Queens College, City University of New York, Flushing, New York
(R.J.B.)
In an effort to correlate the recently cloned MOR-1 receptor with the
pharmacological actions of morphine and morphine-6
-glucuronide (M6G), we have used an antisense paradigm. Rats were injected intracerebroventricularly (i.c.v.) with antisense oligodeoxynucleotides on days 1, 3 and 5 and tested for analgesia on day 6 after
administration of morphine or M6G i.c.v. or after microinjection of
morphine directly into either the periaqueductal gray or the locus
coeruleus. When given i.c.v., the antisense oligodeoxynucleotide
targeting the 5
-untranslated region of exon 1 significantly decreased
the analgesic actions of morphine administered i.c.v. or microinjected directly into the periaqueductal gray or locus coeruleus, with the most
profound inhibition occurring in the periaqueductal gray. Thus,
antisense oligodeoxynucleotides administered into the lateral ventricle
can diffuse into the brainstem and interfere with morphine actions. A
mismatch antisense oligodeoxynucleotide with the same base composition
in which the sequence of four bases was changed was inactive. This same
exon 1 antisense oligodeoxynucleotide, which was active against
morphine analgesia, failed to block M6G analgesia. In contrast,
antisense sequences from exons 2 and 3 decreased M6G, and not morphine,
analgesia. The antisense oligodeoxynucleotide against exon 4 slightly
decreased both morphine and M6G antinociception. These results confirm
the antisense mapping studies on exons 1, 2 and 3 of MOR-1 in mice,
which implied the presence of a novel µ receptor subtype responsible
for M6G analgesia that may represent a splice variant of MOR-1. Unlike
in mice, the probe against exon 4 had a small effect on M6G analgesia.
This article has been cited by other articles:
|
|
 |
|
  E. L. A. van Dorp, R. Romberg, E. Sarton, J. G. Bovill, and A. Dahan Morphine-6-Glucuronide: Morphine's Successor for Postoperative Pain Relief? Anesth. Analg., June 1, 2006; 102(6): 1789 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Goumon, A. Muller, E. Glattard, C. Marban, C. Gasnier, J.-M. Strub, S. Chasserot-Golaz, O. Rohr, G. B. Stefano, I. D. Welters, et al. Identification of Morphine-6-glucuronide in Chromaffin Cell Secretory Granules J. Biol. Chem., March 24, 2006; 281(12): 8082 - 8089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Platt, J. K. Rowlett, S. Izenwasser, and R. D. Spealman Opioid Partial Agonist Effects of 3-O-Methylnaltrexone in Rhesus Monkeys J. Pharmacol. Exp. Ther., March 1, 2004; 308(3): 1030 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Romberg, E. Sarton, L. Teppema, H. W. D. Matthes, B. L. Kieffer, and A. Dahan Comparison of morphine-6-glucuronide and morphine on respiratory depressant and antinociceptive responses in wild type and {micro}-opioid receptor deficient mice Br. J. Anaesth., December 1, 2003; 91(6): 862 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Bolan, R. J. Tallarida, and G. W. Pasternak Synergy between {micro} Opioid Ligands: Evidence for Functional Interactions among {micro} Opioid Receptor Subtypes J. Pharmacol. Exp. Ther., November 1, 2002; 303(2): 557 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-e. Wu, H. Mizoguchi, M. Terashvili, R. J. Leitermann, K.-c. Hung, J. M. Fujimoto, and L. F. Tseng Spinal Pretreatment with Antisense Oligodeoxynucleotides against Exon-1, -4, or -8 of {micro}-Opioid Receptor Clone Leads to Differential Loss of Spinal Endomorphin-1-and Endomorphin-2-Induced Antinociception in the Mouse J. Pharmacol. Exp. Ther., November 1, 2002; 303(2): 867 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Bowen, B. D. Fischer, N. K. Mello, and S. S. Negus Antagonism of the Antinociceptive and Discriminative Stimulus Effects of Heroin and Morphine by 3-Methoxynaltrexone and Naltrexone in Rhesus Monkeys J. Pharmacol. Exp. Ther., July 1, 2002; 302(1): 264 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cherny, C. Ripamonti, J. Pereira, C. Davis, M. Fallon, H. McQuay, S. Mercadante, G. Pasternak, and V. Ventafridda Strategies to Manage the Adverse Effects of Oral Morphine: An Evidence-Based Report J. Clin. Oncol., May 1, 2001; 19(9): 2542 - 2554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Campbell, W. L. Dewey, and S. P. Welch Comparison of [3H]Glyburide Binding with Opiate Analgesia, Tolerance, and Dependence in ICR and Swiss-Webster Mice J. Pharmacol. Exp. Ther., December 1, 2000; 295(3): 1112 - 1119. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. J. Sim-Selley, D. E. Selley, L. J. Vogt, S. R. Childers, and T. J. Martin Chronic Heroin Self-Administration Desensitizes {micro} Opioid Receptor-Activated G-Proteins in Specific Regions of Rat Brain J. Neurosci., June 15, 2000; 20(12): 4555 - 4562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Rady, B. B. Holmes, P. S. Portoghese, and J. M. Fujimoto Morphine Tolerance in Mice Changes Response of Heroin from {micro} to {delta} Opioid Receptors Experimental Biology and Medicine, June 1, 2000; 224(2): 93 - 101. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Fallon Molecular genetics and palliative medicine: what is the link? Palliative Medicine, June 1, 2000; 14(4): 255 - 256. [PDF]
|
|
|
|
 |
|
 Y.-X. Pan, J. Xu, E. Bolan, C. Abbadie, A. Chang, A. Zuckerman, G. Rossi, and G. W. Pasternak Identification and Characterization of Three New Alternatively Spliced {micro}-Opioid Receptor Isoforms Mol. Pharmacol., August 1, 1999; 56(2): 396 - 403. [Abstract] [Full Text]
|
|
|
|
|
|
L. Leventhal, R. M. Silva, G. C. Rossi, G. W. Pasternak, and R. J. Bodnar Morphine-6beta -Glucuronide-Induced Hyperphagia: Characterization of Opioid Action By Selective Antagonists and Antisense Mapping in Rats J. Pharmacol. Exp. Ther., November 1, 1998; 287(2): 538 - 544. [Abstract] [Full Text]
|
|
|
|
|
|
G. P. Brown, K. Yang, O. Ouerfelli, K. M. Standifer, D. Byrd, and G. W. Pasternak 3H-Morphine-6beta -Glucuronide Binding in Brain Membranes and an MOR-1-Transfected Cell Line J. Pharmacol. Exp. Ther., September 1, 1997; 282(3): 1291 - 1297. [Abstract] [Full Text]
|
|
|
|
|
|
L. Leventhal, L. B. Stevens, G. C. Rossi, G. W. Pasternak, and R. J. Bodnar Antisense Mapping of the MOR-1 Opioid Receptor Clone: Modulation of Hyperphagia Induced by DAMGO J. Pharmacol. Exp. Ther., September 1, 1997; 282(3): 1402 - 1407. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
