JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toddywalla, V. S.
Right arrow Articles by Neville, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toddywalla, V. S.
Right arrow Articles by Neville, M. C.

Vol. 280, Issue 2, 669-676, 1997

Active Transport of Nitrofurantoin Across a Mouse Mammary Epithelial Monolayer1

Villi S. Toddywalla , Frank W. Kari and Margaret C. Neville

Department of Physiology, University of Colorado Health Sciences Center (M.C.N., V.S.T.), Denver, Colorado, Institute for Research in Reproduction, Indian Council of Medical Research (V.S.T.), Bombay, India and National Institute of Environmental Health Sciences (F.W.K.), Research Triangle Park, North Carolina

The antibiotic nitrofurantoin is transported against an electrochemical gradient into milk.A monolayer of CIT3 cells, a subline of the Comma 1D normal mouse mammary epithelial cell line, transports [14C]-nitrofurantoin against a concentration gradient from the basal to the apical solution when grown on membrane filters. In a side-by-side diffusion chamber with well-stirred solutions on both sides, the transfer rate is 50% higher in the basal-to-apical than in the apical-to-basal direction. Nonlabeled nitrofurantoin (500 µM) in the basal chamber equalized the transport in both directions, suggesting that a specific transporter is responsible for the basal-to-apical increment in flux. From inhibition studies, the apparent affinity of this transporter for nitrofurantoin is 50 µM. Changes in pH between 6.4 and 7.8 had no effect on the active transport component of the flux but did affect the passive flux component. Passive flux of the nonionized molecule was 2.6 times faster than that of the ionized molecule, but the ionized molecule did appear to cross the membrane passively. Our findings show that nitrofurantoin is actively transported across a mammary epithelial cell monolayer by a transporter whose affinity for nitrofurantoin does not depend on the anionic charge on nitrofurantoin. The pH dependence of a parallel passive pathway suggests that both nonionized and ionized forms of nitrofurantoin cross the membranes of the mammary epithelial cell by passive diffusion.

Copyright © by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Drug Metab. Dispos.Home page
L. Wang, M. Leggas, M. Goswami, P. E. Empey, and P. J. McNamara
N-(4-[2-(1,2,3,4-Tetrahydro-6,7-dimethoxy-2-isoquinolinyl)ethyl]-phenyl)-9,10-dihydro-5-methoxy-9-oxo-4-acridine Carboxamide (GF120918) As a Chemical ATP-Binding Cassette Transporter Family G Member 2 (Abcg2) Knockout Model to Study Nitrofurantoin Transfer into Milk
Drug Metab. Dispos., December 1, 2008; 36(12): 2591 - 2596.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Fischer, H. Stuckas, M. Gluth, T. D. Russell, M. C. Rudolph, N. E. Beeman, S. Bachmann, S. Umemura, Y. Ohashi, M. C. Neville, et al.
Impaired tight junction sealing and precocious involution in mammary glands of PKN1 transgenic mice
J. Cell Sci., July 1, 2007; 120(13): 2272 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. Merino, J. W. Jonker, E. Wagenaar, A. E. van Herwaarden, and A. H. Schinkel
The Breast Cancer Resistance Protein (BCRP/ABCG2) Affects Pharmacokinetics, Hepatobiliary Excretion, and Milk Secretion of the Antibiotic Nitrofurantoin
Mol. Pharmacol., May 1, 2005; 67(5): 1758 - 1764.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
P. M. Gerk, J. A. Moscow, and P. J. McNamara
BASOLATERAL ACTIVE UPTAKE OF NITROFURANTOIN IN THE CIT3 CELL CULTURE MODEL OF LACTATION
Drug Metab. Dispos., June 1, 2003; 31(6): 691 - 693.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. B. Shennan and M. Peaker
Transport of Milk Constituents by the Mammary Gland
Physiol Rev, July 1, 2000; 80(3): 925 - 951.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
F. W. Kari, R. Weaver, and M. C. Neville
Active Transport of Nitrofurantoin Across the Mammary Epithelium In Vivo
J. Pharmacol. Exp. Ther., February 1, 1997; 280(2): 664 - 668.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1997 by the American Society for Pharmacology and Experimental Therapeutics.