![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 280, Issue 1, 428-438, 1997
7 Nicotinic
Acetylcholine Receptor Significantly Raises Intracellular Free Calcium
Departments of
Physiology and Pharmacology (O.D., M.L.M.) and
Internal Medicine (Gerontology) (O.D., M.R.), Bowman Gray School of
Medicine of the Wake Forest University, Winston-Salem, North Carolina
and
Neuroscience Research (D-47W) (M.G., L.M.M., J.P.S.), Abbott
Laboratories, Abbott Park, Illinois
The 
7 nicotinic acetylcholine receptor (nAChR) subtype,
unlike other neuronal nicotinic receptors, exhibits a relatively high
permeability to Ca++ ions. Although Ca++ entry
through this receptor subtype has been implicated in various Ca++-dependent processes in the central nervous system,
little is known about how this receptor modulates mammalian
intracellular Ca++ dynamics. Intracellular Ca++
responses evoked by activation of the human 7 nAChRs
stably expressed in HEK-293 (human embryonic kidney) cells were
studied. Inward current and intracellular Ca++ transients
were recorded simultaneously in response to a fast drug application
system. Current recordings under whole-cell voltage-clamp and fast
ratiometric intracellular Ca++ imaging acquisition were
synchronized to drug pulses. The mean peak
[Ca++]i observed with 100 µM (
)-nicotine
was 356 ± 48 nM (n = 8). The magnitude of the
intracellular Ca++ elevation corresponds to a 20%
fractional current carried by Ca++ ions. The
EC50 of the intracellular Ca++ responses for
()-nicotine, (±)-epibatidine, 1,1 dimethyl-4-phenyl-piperazinium and
acetylcholine were 51, 3.5, 75 and 108 µM, respectively. These EC50 values strongly correlate with those recorded for the
cationic inward current through 7 nAChR.
-Bungarotoxin, methyllcaconitine or extracellular Ca++
chelation ablated ()-nicotine-evoked increase in intracellular Ca++ concentration. This study provides evidence that
cation influx through the human 7 nAChR is sufficient to
mediate a significant, transient, rise in intracellular
Ca++ concentration.
This article has been cited by other articles:
|
|
 |
|
  J. Arredondo, A. I. Chernyavsky, D. L. Jolkovsky, K. E. Pinkerton, and S. A. Grando Receptor-mediated tobacco toxicity: acceleration of sequential expression of {alpha}5 and {alpha}7 nicotinic receptor subunits in oral keratinocytes exposed to cigarette smoke FASEB J, May 1, 2008; 22(5): 1356 - 1368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Guo and R. A. J. Lester Ca2+ Flux and Signaling Implications by Nicotinic Acetylcholine Receptors in Rat Medial Habenula J Neurophysiol, January 1, 2007; 97(1): 83 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Razani-Boroujerdi and M. L. Sopori Early Manifestations of NNK-Induced Lung Cancer: Role of Lung Immunity in Tumor Susceptibility Am. J. Respir. Cell Mol. Biol., January 1, 2007; 36(1): 13 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Arredondo, A. I. Chernyavsky, D. L. Jolkovsky, K. E. Pinkerton, and S. A. Grando Receptor-mediated tobacco toxicity: cooperation of the Ras/Raf-1/MEK1/ERK and JAK-2/STAT-3 pathways downstream of {alpha}7 nicotinic receptor in oral keratinocytes FASEB J, October 1, 2006; 20(12): 2093 - 2101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Tournier, K. Maouche, C. Coraux, J.-M. Zahm, I. Cloez-Tayarani, B. Nawrocki-Raby, A. Bonnomet, H. Burlet, F. Lebargy, M. Polette, et al. {alpha}3{alpha}5{beta}2-Nicotinic Acetylcholine Receptor Contributes to the Wound Repair of the Respiratory Epithelium by Modulating Intracellular Calcium in Migrating Cells Am. J. Pathol., January 1, 2006; 168(1): 55 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bray, J.-H. Son, P. Kumar, and S. Meizel Mice Deficient in CHRNA7, a Subunit of the Nicotinic Acetylcholine Receptor, Produce Sperm with Impaired Motility Biol Reprod, October 1, 2005; 73(4): 807 - 814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Diaz-Hernandez, J. Sanchez-Nogueiro, J. Pintor, and M. T. Miras-Portugal Interaction between Dinucleotide and Nicotinic Receptors in Individual Cholinergic Terminals J. Pharmacol. Exp. Ther., December 1, 2004; 311(3): 954 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Son and S. Meizel Evidence Suggesting That the Mouse Sperm Acrosome Reaction Initiated by the Zona Pellucida Involves an {alpha}7 Nicotinic Acetylcholine Receptor Biol Reprod, April 1, 2003; 68(4): 1348 - 1353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bray, J.-H. Son, and S. Meizel A Nicotinic Acetylcholine Receptor Is Involved in the Acrosome Reaction of Human Sperm Initiated by Recombinant Human ZP3 Biol Reprod, September 1, 2002; 67(3): 782 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Francis, E. Y. Cheng, G. A. Weiland, and R. E. Oswald Specific Activation of the alpha 7 Nicotinic Acetylcholine Receptor by a Quaternary Analog of Cocaine Mol. Pharmacol., July 1, 2001; 60(1): 71 - 79. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Nguyen, A Ndoye, L. Hall, S Zia, J Arredondo, A. Chernyavsky, D. Kist, B. Zelickson, M. Lawry, and S. Grando Programmed cell death of keratinocytes culminates in apoptotic secretion of a humectant upon secretagogue action of acetylcholine J. Cell Sci., January 3, 2001; 114(6): 1189 - 1204. [Abstract] [PDF]
|
|
|
|
|
|
S. Zia, A. Ndoye, T. X. Lee, R. J. Webber, and S. A. Grando Receptor-Mediated Inhibition of Keratinocyte Migration by Nicotine Involves Modulations of Calcium Influx and Intracellular Concentration J. Pharmacol. Exp. Ther., June 1, 2000; 293(3): 973 - 981. [Abstract] [Full Text]
|
|
|
|
|
|
V. Gerzanich, F. Wang, A. Kuryatov, and J. Lindstrom alpha 5 Subunit Alters Desensitization, Pharmacology, Ca++ Permeability and Ca++ Modulation of Human Neuronal alpha 3 Nicotinic Receptors J. Pharmacol. Exp. Ther., July 1, 1998; 286(1): 311 - 320. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Ragozzino, B. Barabino, S. Fucile, and F. Eusebi Ca2+ permeability of mouse and chick nicotinic acetylcholine receptors expressed in transiently transfected human cells J. Physiol., March 15, 1998; 507(3): 749 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Krause, B. Buisson, S. Bertrand, P.-J. Corringer, J.-L. Galzi, J.-P. Changeux, and D. Bertrand Ivermectin: A Positive Allosteric Effector of the alpha 7 Neuronal Nicotinic Acetylcholine Receptor Mol. Pharmacol., February 1, 1998; 53(2): 283 - 294. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Palma, L. Maggi, F. Eusebi, and R. Miledi Neuronal nicotinic threonine-for-leucine 247 alpha 7 mutant receptors show different gating kinetics when activated by acetylcholine or by the noncompetitive agonist 5-hydroxytryptamine PNAS, September 2, 1997; 94(18): 9915 - 9919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fucile, E. Palma, A. M. Mileo, R. Miledi, and F. Eusebi Human neuronal threonine-for-leucine-248 alpha 7 mutant nicotinic acetylcholine receptors are highly Ca2+ permeable PNAS, March 28, 2000; 97(7): 3643 - 3648. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
