JPET Celsis microsomes equal better data

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Castaño, E.
Right arrow Articles by Gil, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Castaño, E.
Right arrow Articles by Gil, J.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*ACETYLSALICYLIC ACID

Vol. 280, Issue 1, 366-372, 1997

Inhibition of DNA Synthesis by Aspirin in Swiss 3T3 Fibroblasts1

Esther Castaño, Mireia Dalmau, Mercè Martí, Félix Berrocal, Ramon Bartrons and Joan Gil

Unitat de Bioquímica, Departament de Ciències Fisiològiques, Campus de Bellvitge, Universitat de Barcelona, L'Hospitalet (E.C., M.D., R.B., J.G.) and Química Farmacéutica Bayer S.A. Division Consumer Care. Barcelona, Spain (M.M., F.B.)

In Swiss 3T3 fibroblasts, aspirin inhibited proliferation induced by the complete mitogenic factors platelet-derived growth factor (PDGF) and bombesin. Aspirin decreased the maximum mitogenic effect of bombesin without modifying the concentration necessary to obtain half maximal DNA synthesis stimulation. In contrast, aspirin only decreased mitogenesis at subsaturating PDGF concentrations. The effect of aspirin was found to be concentration dependent. The half-maximal effect occurred at approximately 150 µM. The maximal inhibition was obtained when aspirin was added during the first hour after growth factor addition. At this time, both PDGF and bombesin induced prostaglandin E2 synthesis. PDGF induced much higher levels of prostaglandin E2 than bombesin. The inhibitory effects of aspirin on PDGF or bombesin-stimulated DNA synthesis were counteracted by 280 nM prostaglandin E2. Aspirin effects were overcome by agents that increase cellular cyclic adenosine monophosphate levels but not by activation of protein kinase C. The significance of the antiproliferative action of aspirin might be associated with epidemiological data that show a reduced incidence of colorectal and other cancers after aspirin treatment.

Copyright © by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Toxicol SciHome page
K. E. Eblin, T. G. Bredfeldt, S. Buffington, and A. J. Gandolfi
Mitogenic Signal Transduction Caused by Monomethylarsonous Acid in Human Bladder Cells: Role in Arsenic-Induced Carcinogenesis
Toxicol. Sci., February 1, 2007; 95(2): 321 - 330.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
K. J. Trouba and D. R. Germolec
Micromolar Concentrations of Sodium Arsenite Induce Cyclooxygenase-2 Expression and Stimulate p42/44 Mitogen-Activated Protein Kinase Phosphorylation in Normal Human Epidermal Keratinocytes
Toxicol. Sci., June 1, 2004; 79(2): 248 - 257.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
E. Castaño, R. Bartrons, and J. Gil
Inhibition of Cyclooxygenase-2 Decreases DNA Synthesis Induced by Platelet-Derived Growth Factor in Swiss 3T3 Fibroblasts
J. Pharmacol. Exp. Ther., May 1, 2000; 293(2): 509 - 513.
[Abstract] [Full Text]

Home page
 BloodHome page
B. Bellosillo, M. Pique, M. Barragan, E. Castano, N. Villamor, D. Colomer, E. Montserrat, G. Pons, and J. Gil
Aspirin and Salicylate Induce Apoptosis and Activation of Caspases in B-Cell Chronic Lymphocytic Leukemia Cells
Blood, August 15, 1998; 92(4): 1406 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-S. Guo, M. R. Hellmich, X. D. Wen, and C. M. Townsend Jr.
Activator Protein-1 Transcription Factor Mediates Bombesin-stimulated Cyclooxygenase-2 Expression in Intestinal Epithelial Cells
J. Biol. Chem., June 15, 2001; 276(25): 22941 - 22947.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1997 by the American Society for Pharmacology and Experimental Therapeutics.