![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
LG Aguayo, JC Tapia and FC Pancetti
Department of Physiology, University of Concepcion, Chile.
Ethanol (1-200 mM), a potent depressor of respiration and motor activity, potentiated the inhibitory Cl- current activated by glycine in 80% of the cultured mouse spinal (n = 236) neurons studied. Ethanol (100 mM) had no effect on the gamma-aminobutyric acidA current and slightly inhibited the N-methyl-D-aspartate current in these neurons. Ethanol increased the affinity of the receptors to glycine without changing the maximal amplitude of the glycine current. The EC50 was reduced from 54 +/- 3 microM in the absence of ethanol to 38 +/- 5 microM in the presence of ethanol. Activation of GTP binding proteins in the neurons with intracellular guanosine-5'-0-(2-thiotriiphosphate) (0.5 mM) enhanced the effect of ethanol, and application of a similar concentration of guanosine 5'-0-(2-thiodiphosphate had an inhibitory effect upon the current potentiation. The potentiating effect of ethanol persisted after culturing the neurons with pertussis toxin, but not with cholera toxin, an irreversible activator of Gs. Activation of cyclic AMP-dependent protein kinase by cyclic AMP and Sp-adenosine- 3',5'-cyclic monophosphothioate triethylamine salt, but not of protein kinase C and protein kinase G, potentiated the glycine current. The effect of Sp-adenosine-3',5'-cyclic monophosphothioate triethylamine salt, but not of ethanol, was inhibited completely by the protein kinase A peptide inhibitor. These results suggest that ethanol potentiates the glycine activated Cl- current by modifying a signal transduction step other than protein kinase A.
This article has been cited by other articles:
|
|
 |
|
  G. E. Yevenes, G. Moraga-Cid, R. W. Peoples, G. Schmalzing, and L. G. Aguayo A selective G{beta}{gamma}-linked intracellular mechanism for modulation of a ligand-gated ion channel by ethanol PNAS, December 23, 2008; 105(51): 20523 - 20528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dubois, H. Houchi, M. Naassila, M. Daoust, and O. Pierrefiche Blunted response to low oxygen of rat respiratory network after perinatal ethanol exposure: involvement of inhibitory control J. Physiol., March 1, 2008; 586(5): 1413 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Lynch Molecular Structure and Function of the Glycine Receptor Chloride Channel Physiol Rev, October 1, 2004; 84(4): 1051 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Lobo, M. P. Mascia, J. R. Trudell, and R. A. Harris Channel Gating of the Glycine Receptor Changes Accessibility to Residues Implicated in Receptor Potentiation by Alcohols and Anesthetics J. Biol. Chem., August 6, 2004; 279(32): 33919 - 33927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. D. Eggers and A. J. Berger Mechanisms for the Modulation of Native Glycine Receptor Channels by Ethanol J Neurophysiol, June 1, 2004; 91(6): 2685 - 2695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Sebe, E. D. Eggers, and A. J. Berger Differential Effects of Ethanol on GABAA and Glycine Receptor-Mediated Synaptic Currents in Brain Stem Motoneurons J Neurophysiol, August 1, 2003; 90(2): 870 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tao and J. H. Ye Protein Kinase C Modulation of Ethanol Inhibition of Glycine-Activated Current in Dissociated Neurons of Rat Ventral Tegmental Area J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 967 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Ye, L. Tao, L. Zhu, and J. J. McArdle Ethanol Inhibition of Glycine-Activated Responses in Neurons of Ventral Tegmental Area of Neonatal Rats J Neurophysiol, November 1, 2001; 86(5): 2426 - 2434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Ye, L. Tao, J. Ren, R. Schaefer, P. L. Liu, D. A. Schiller, and J. J. McArdle Ethanol Potentiation of Glycine-Induced Responses in Dissociated Neurons of Rat Ventral Tegmental Area J. Pharmacol. Exp. Ther., January 1, 2001; 296(1): 77 - 83. [Abstract] [Full Text]
|
|
|
|
|
|
E. D. Eggers, J. A. O'Brien, and A. J. Berger Developmental Changes in the Modulation of Synaptic Glycine Receptors by Ethanol J Neurophysiol, November 1, 2000; 84(5): 2409 - 2416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. van Zundert, F. A. Albarran, and L. G. Aguayo Effects of Chronic Ethanol Treatment on gamma -Aminobutyric AcidA and Glycine Receptors in Mouse Glycinergic Spinal Neurons J. Pharmacol. Exp. Ther., October 1, 2000; 295(1): 423 - 429. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. E. Wong, D. L. Tauck, E. G. Fong, and J. J. Kendig Glutamate Receptor-Mediated Hyperexcitability after Ethanol Exposure in Isolated Neonatal Rat Spinal Cord J. Pharmacol. Exp. Ther., April 1, 1998; 285(1): 201 - 207. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
