JPET Celsis microsomes equal better data

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chan, B. S.
Right arrow Articles by Duggin, G. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chan, B. S.
Right arrow Articles by Duggin, G. G.

Transport of paraquat in a renal epithelial cell line LLC-PK1

BS Chan, VA Lazzaro, JP Seale and GG Duggin

Renal Laboratory, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.

Transport of paraquat (PQ), a cationic herbicide, was investigated in a proximal renal epithelial cell line, LLC-PK1. Collagencoated permeable filters were used to study the direction of PQ transport. PQ was transported predominantly from the basolateral to apical (B-->A) membrane of these cells. The B-->A flux and uptake of PQ were saturable with time and increasing concentrations, energy dependent and inhibited by several cations. Quinine was the most potent inhibitor of basolateral PQ uptake, followed by cimetidine and then tetraethylammonium acetate (P < .0001). The noninhibitable basolateral uptake of PQ has an apparent K(m) of 357 microM and a Vmax of 1.47 pmol/micrograms protein/2 min. For flux studies, only quinine inhibited the B-->A flux of PQ (P = .02). Putrescine, p-aminohippurate, probenecid, N-methylnicotinamide and choline did not inhibit the flux or uptake of PQ. 5-N,N-Hexamethylene amiloride, a cationic amiloride analog and a potent inhibitor of the Na/H exchanger, significantly inhibited the uptake of PQ from either side (P < .0001). Acidic pH in the apical medium inhibited the uptake of PQ from either side. The studies demonstrated that PQ was actively transported by the LLC-PK1 cells. PQ shared a similar transport system with several cations, which appeared to have a more significant inhibition on the transcellular uptake than the flux of PQ.

Volume 279, Issue 2, pp. 625-632, 11/01/1996
Copyright © 1996 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Chen, S. Zhang, M. Sorani, and K. M. Giacomini
Transport of Paraquat by Human Organic Cation Transporters and Multidrug and Toxic Compound Extrusion Family
J. Pharmacol. Exp. Ther., August 1, 2007; 322(2): 695 - 700.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
R Machaalani, V Lazzaro, and G G Duggin
The characterisation and uptake of paraquat in cultured baboon kidney proximal tubule cells (bPTC)
Human and Experimental Toxicology, February 1, 2001; 20(2): 90 - 99.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1996 by the American Society for Pharmacology and Experimental Therapeutics.