JPET Introducing ALZET?ew Model 2006 Pump

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abood, M. E.
Right arrow Articles by Tao, Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abood, M. E.
Right arrow Articles by Tao, Q.

Characterization of a delta opioid receptor in rat pheochromocytoma cells

ME Abood and Q Tao

Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, USA.

In one subclone of PC12 pheochromocytoma cells, PC12h, the levels of delta opioid receptors markedly increase in response to nerve growth factor (NGF). This increase, as assessed by [3H]diprenorphine binding, is found only under specific culture conditions. NGF treatment of PC12h cells also results in the induction of delta opioid receptor (DOR-1) mRNA. The time course for NGF induction of mRNA and protein is similar, although the levels of mRNA increase approximately 5-fold, whereas the levels of receptor increase only 2-fold. Competition studies with selective delta ([D-Pen2,5]-enkephalin, DPDPE), mu ([D-Ala2,N-Me- Phe4,Gly-ol5]- enkephalin, DAMGO) and kappa (trans-[+/-]-3,4-dichloro-N- methyl-N-[2-(1-pyrrolidinyl)-cyclohexyl]- benzenacetamide methansulfonate salt, U50,488) opioid agonists to displace [3H]diprenorphine confirm that the delta subtype of opioid receptor is present on PC12h cells. The delta opioid receptor is coupled functionally, as indicated by agonist inhibition of forskolin- stimulated cAMP accumulation in a naloxone-reversible manner. Finally, the delta opioid receptor was cloned from PC12h cells. The sequence was identical with that described previously for the rat clone. The PC12h cell line thus provides a model system in which to study regulation of delta opioid receptors.

Volume 274, Issue 3, pp. 1566-1573, 09/01/1995
Copyright © 1995 by American Society for Pharmacology and Experimental Therapeutics






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1995 by the American Society for Pharmacology and Experimental Therapeutics.