JPET Assistant Professor of Medicine (Clinician-Educator)

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khan, I.
Right arrow Articles by Rangachari, P. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khan, I.
Right arrow Articles by Rangachari, P. K.

Messenger RNAs for neurokinin and histamine receptor subtypes in isolated canine colonic crypts

I Khan, N Lapierre and PK Rangachari

Dept. Medicine, McMaster University, Hamilton, Ont., Canada.

Functional subtyping of neurokinin and histamine receptors on the canine proximal colon suggested that different receptors were involved in mediating the neural and non-neural effects on ion transport. If the non-neural effects involved direct stimulation of the transporting epithelial cells, mRNA for specific receptor subtypes should be present in colonic crypt cells. Pharmacological data predicted that RNA from such cells would include mRNAs for neurokinin (NK-1) and histamine (H1 and H2) receptors but not for NK-2 or NK-3 receptors. Using published sequences for the above receptors and reverse-transcription polymerase chain reaction, we have noted that isolated canine colonic crypts had mRNA for NK-1 and NK-2 receptors but not for NK-3 receptors. Messenger RNAs for both H1 and H2 receptors were present. The muscularis mucosa and submucosal plexuses had mRNAs for NK-1, NK-3, H1 and H2 receptors but not for NK-2 receptors. The circular and longitudinal muscles expressed mRNA for all three neurokinin and both histamine receptors. Messenger RNA levels for the alpha-1 isoform of the catalytic subunit of the sodium pump were higher in crypts and lower in muscularis mucosa and muscle preparation. Thus these observations provide further evidence for the existence of different receptor subtypes in the canine colon.

Volume 272, Issue 3, pp. 1285-1292, 03/01/1995
Copyright © 1995 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Riegler, I. Castagliuolo, P. T. C. So, M. Lotz, C. Wang, M. Wlk, T. Sogukoglu, E. Cosentini, G. Bischof, G. Hamilton, et al.
Effects of substance P on human colonic mucosa in vitro
Am J Physiol Gastrointest Liver Physiol, June 1, 1999; 276(6): G1473 - G1483.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. Pothoulakis, I. Castagliuolo, S. E. Leeman, C.-C. Wang, H. Li, B. J. Hoffman, and E. Mezey
Substance P receptor expression in intestinal epithelium in Clostridium difficile toxin A enteritis in rats
Am J Physiol Gastrointest Liver Physiol, July 1, 1998; 275(1): G68 - G75.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1995 by the American Society for Pharmacology and Experimental Therapeutics.