Uptake and phosphorylation of thiamine in rabbit primary proximal tubule cells and Madin Darby canine kidney cells. II. Effect of ethanol

MA Mahajan, M Acara and M Taub

Department of Pharmacology and Therapeutics, School of Medicine and Biomedical Sciences, State University of New York at Buffalo.

Uptake of [14C]thiamine was studied in renal primary proximal tubule cells and in Madin Darby Canine Kidney cells in culture. Findings were compared with data for the accumulation of [14C]thiamine and its phosphorylation in renal cortical slices. There was a saturable component for thiamine uptake in all cell types. When normalized for milligrams of protein, renal cortical slices accumulated 82% less 14C than renal primary proximal tubule cells and 51% less than distal tubule cells. Maximal [14C]thiamine levels accumulated by the saturable component was 1.33 nmol/g in slices and 7.02 nmol/g in proximal tubule cells. Ethanol, at 25 mM, inhibited thiamine uptake and phosphorylation in the cell cultures similar to its effect in the kidney tissue.

Volume 268, Issue 3, pp. 1316-1320, 03/01/1994
Copyright © 1994 by American Society for Pharmacology and Experimental Therapeutics