![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
JG Granneman
Department of Psychiatry, Wayne State University School of Medicine, Detroit, Michigan.
The coupling of beta 1 and beta 3 adrenergic receptors to adenylyl cyclase was examined in membranes of isolated white adipocytes. The activation of adenylyl cyclase by isoproterenol (ISO) was biphasic. The high-affinity activation of adenylyl cyclase, which occurred at submicromolar concentrations of ISO, was mediated by beta 1 receptors, whereas low-affinity activation was mediated by beta 3 receptors. The relative activation of adenylyl cyclase by beta 3 receptors was less when ISO was used to stimulate activity, compared to when the beta 3- selective agonist BRL 37344 was used. These data indicate that both receptor subtypes can stimulate the same adenylyl cyclase in membranes of control cells, and that the activation of adenylyl cyclase by beta 1 receptors by low concentrations of catecholamines can obscure the activation by beta 3 receptors by high concentrations of catecholamines. Exposure of adipocytes to ISO at concentrations that either selectively stimulate beta 1 receptors or nonselectively stimulate both beta receptor subtypes greatly decreased the ability of beta 1, but not beta 3, receptors to activate adenylyl cyclase. In contrast, the exposure of cells to the beta 3-selective agonist BRL only slightly desensitized beta 1 receptors and did not affect beta 3 receptor activation of adenylyl cyclase. These data indicate that acute agonist exposure desensitizes beta 1, but not beta 3, receptors.
This article has been cited by other articles:
|
|
 |
|
  G. D'Angelo, J. D. Mintz, J. E. Tidwell, A. M. Schreihofer, D. M. Pollock, and D. W. Stepp Exaggerated Cardiovascular Stress Responses and Impaired {beta}-Adrenergic-Mediated Pressor Recovery in Obese Zucker Rats Hypertension, December 1, 2006; 48(6): 1109 - 1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Martins-Afferri, W. T. L. Festuccia, L. C. C. Navegantes, M. A. R. Garofalo, L. M. Botion, I. C. Kettelhut, and R. H. Migliorini Response to Intra- and Extracellular Lipolytic Agents and Hormone-Sensitive Lipase Translocation Are Impaired in Adipocytes from Rats Adapted to a High-Protein, Carbohydrate-Free Diet J. Nutr., November 1, 2004; 134(11): 2919 - 2923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hatakeyama, Y. Sakata, S. Takakura, T. Manda, and S. Mutoh Acute and chronic effects of FR-149175, a {beta}3-adrenergic receptor agonist, on energy expenditure in Zucker fatty rats Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R336 - R341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pelat, P. Verwaerde, J. Galitzky, M. Lafontan, M. Berlan, J.-M. Senard, and J.-L. Montastruc High Isoproterenol Doses Are Required to Activate beta 3-Adrenoceptor-Mediated Functions in Dogs J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 246 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Bosch, A. C. Schneck, J. Kiehn, W. Zhang, A. Hambrock, B. W. Eigenberger, N. Rub, J. Gogel, C. Mewis, L. Seipel, et al. {beta}3-Adrenergic regulation of an ion channel in the heart--inhibition of the slow delayed rectifier potassium current IKs in guinea pig ventricular myocytes Cardiovasc Res, December 1, 2002; 56(3): 393 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Marion-Latard, I. De Glisezinski, F. Crampes, M. Berlan, J. Galitzky, H. Suljkovicova, D. Riviere, and V. Stich A single bout of exercise induces {beta}-adrenergic desensitization in human adipose tissue Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R166 - R173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chaudhry and J. G. Granneman Differential regulation of functional responses by beta -adrenergic receptor subtypes in brown adipocytes Am J Physiol Regulatory Integrative Comp Physiol, July 1, 1999; 277(1): R147 - R153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Kumar, G. D. Sunvold, and P. J. Scarpace Dietary vitamin A supplementation in rats: suppression of leptin and induction of UCP1 mRNA J. Lipid Res., May 1, 1999; 40(5): 824 - 829. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. J. Scarpace and M. Matheny Leptin induction of UCP1 gene expression is dependent on sympathetic innervation Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E259 - E264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Jockers, T. Issad, V. Zilberfarb, P. de Coppet, S. Marullo, and A. D. Strosberg Desensitization of the {beta}-Adrenergic Response in Human Brown Adipocytes Endocrinology, June 1, 1998; 139(6): 2676 - 2684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Granneman, K. N. Lahners, and Y. Zhai Agonist Interactions with Chimeric and Mutant beta 1- and beta 3-Adrenergic Receptors: Involvement of the Seventh Transmembrane Region in Conferring Subtype Specificity Mol. Pharmacol., May 1, 1998; 53(5): 856 - 861. [Abstract] [Full Text]
|
|
|
|
|
|
X. Liu, F. Perusse, and L. J. Bukowiecki Mechanisms of the antidiabetic effects of the beta 3-adrenergic agonist CL-316243 in obese Zucker-ZDF rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1212 - R1219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Atgie, F. D'Allaire, and L. J. Bukowiecki Role of beta 1- and beta 3-adrenoceptors in the regulation of lipolysis and thermogenesis in rat brown adipocytes Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1136 - C1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Granneman, Y. Zhai, and K. N. Lahners Selective Up-Regulation of alpha 1a-Adrenergic Receptor Protein and mRNA in Brown Adipose Tissue by Neural and beta 3-Adrenergic Stimulation Mol. Pharmacol., April 1, 1997; 51(4): 644 - 650. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. S. Susulic, R. C. Frederich, J. Lawitts, E. Tozzo, B. B. Kahn, M.-E. Harper, J. Himms-Hagen, J. S. Flier, and B. B. Lowell Targeted Disruption of the beta(3)-Adrenergic Receptor Gene J. Biol. Chem., December 8, 1995; 270(49): 29483 - 29492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Klaus, L Choy, O Champigny, A. Cassard-Doulcier, S Ross, B Spiegelman, and D Ricquier Characterization of the novel brown adipocyte cell line HIB 1B. Adrenergic pathways involved in regulation of uncoupling protein gene expression J. Cell Sci., January 1, 1994; 107(1): 313 - 319. [Abstract] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
