![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
YN Kwok, C McIntosh and J Brown
Department of Physiology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
The effect of adenosine, adenosine 5'-triphosphate (ATP) and ATP analogs on basal gastric somatostatin-like immunoreactivity (SLI) release was studied using the vascularly perfused rat stomach. The release of gastric SLI was stimulated by adenosine (0.6-60 microM) concentration dependently, while the release of immunoreactive gastrin was inhibited by 1 and 10 microM adenosine. The stimulatory action of adenosine was probably mediated by adenosine receptors because the receptor antagonist, 8-phenyltheophylline, abolished the action of adenosine. In addition, the adenosine-induced release of SLI was not mediated by a cholinergic or beta-adrenergic mechanism, since atropine, hexamethonium or propranolol did not block the action of adenosine. Dipyridamole enhanced the adenosine-stimulated, but not 2- chloroadenosine-induced SLI release. Since the adenosine analog, 2- chloroadenosine, is resistant to the adenosine uptake mechanism, it is likely that adenosine-stimulated release of gastric SLI is due to the activation of extracellular receptors. ATP also stimulated gastric SLI release. The analogs alpha,beta-methyleneadenosine triphosphate and diphosphate, which are resistant to metabolic breakdown, did not stimulate basal SLI release, while gamma,beta-methyleneadenosine triphosphate, which can be metabolized, increased the release of SLI. In addition, the ATP-induced release of SLI was abolished by 8- phenyltheophylline. Therefore, the action of ATP is likely to be a result of its metabolism to adenosine.
This article has been cited by other articles:
|
|
 |
|
  G. K. Yang, J.-F. Chen, T. J. Kieffer, and Y. N. Kwok Regulation of Somatostatin Release by Adenosine in the Mouse Stomach J. Pharmacol. Exp. Ther., May 1, 2009; 329(2): 729 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yip, H. C. H. Leung, and Y. N. Kwok Effect of Omeprazole on Gastric Adenosine A1 and A2A Receptor Gene Expression and Function J. Pharmacol. Exp. Ther., October 1, 2004; 311(1): 180 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yip, H. C. H. Leung, and Y. N. Kwok Role of Adenosine A1 Receptor in the Regulation of Gastrin Release J. Pharmacol. Exp. Ther., August 1, 2004; 310(2): 477 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yip and Y. N. Kwok Role of Adenosine A2A Receptor in the Regulation of Gastric Somatostatin Release J. Pharmacol. Exp. Ther., May 1, 2004; 309(2): 804 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. H. Percy, J. M. Warren, and J. T. Brunz Characteristics of the muscularis mucosae in the acid-secreting region of the rabbit stomach Am J Physiol Gastrointest Liver Physiol, May 1, 1999; 276(5): G1213 - G1220. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
