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KE Thummel, JT Slattery and SD Nelson
Department of Pharmaceutics, University of Washington, Seattle.
The effect of 5 mM ethanol, maintained by a constant infusion, on the hepatotoxicity and disposition of acetaminophen was examined in 3- methylcholanthrene-induced rats. Ethanol infusion, which began 1 hr before acetaminophen and was maintained for 9 hr, resulted in a 2.8- fold higher hepatic glutathione concentration 6 hr after acetaminophen than did saline infusion. Ethanol infusion also diminished the rise in the 24 hr postacetaminophen plasma alanine transferase concentration by approximately 46%. Ethanol (5 mM) had only a modest effect on the oxidation of acetaminophen in rat liver microsomes, 7 to 14% inhibition over a range of acetaminophen concentration of 0.1 to 3 mM, whereas a 30 to 40% decline of covalent binding of acetaminophen-derived material was observed in vivo (peak acetaminophen concentration approximately 3 mM). Thus, a mechanism other than direct inhibition of cytochrome(s) P- 450 by ethanol is invoked to account for the protective effect of ethanol. Ethanol infusion increased the ratio of total hepatocellular NADH/NAD+, and the ratio of free NADH/NAD+ in cytosol and mitochondria as a consequence of sequential oxidations of ethanol producing acetaldehyde and acetic acid in the respective compartments. The toxic electrophile produced by cytochrome P-450 oxidation of acetaminophen, N- acetyl-p-benzoquinoneimine, is reduced rapidly by NADH in aqueous solution. However, acetaminophen alone also increased free NADH/NAD+ in cytosol, and there was no indication that N-acetyl-p-benzoquinoneimine consumed NADH generated by the oxidation of ethanol. Ethanol infusion also increased the ratio of total hepatocellular NADPH/NADP+, apparently through transhydrogenation of NADP+ by NADH in mitochondria.(ABSTRACT TRUNCATED AT 250 WORDS)
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