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Effects of chronic ethanol treatment on membrane potential, its electrogenic pump component and Na-K pump activity of cultured rat skeletal myotubes

C Brodie and SR Sampson

Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.

Effects of chronic ethanol treatment were examined on transmembrane resting potential (Em), electrogenic pump component of Em and on Na-K pump activity of cultured rat skeletal myotubes. Properties of Na-K pump activity were determined by measurement of [3H]ouabain binding and 86Rb uptake by myotubes after incubation of myotubes in ethanol (217 mM) for up to 9 days. Chronic ethanol exposure caused an increase in Em, an increase in electrogenic pump component of Em and a decrease in depolarizing response to addition of ethanol to the myotubes. Chronic ethanol also produced increases in Na-K pump sites and in Na-K pump activity. Thus, chronic ethanol treatment causes a compensatory increase in amount and activity of the Na-K pump, and this results in an increased expression of the electrogenic pump component of Em. These effects may represent the membrane expression of tolerance to the depressant effects of ethanol.

Volume 242, Issue 3, pp. 1104-1108, 09/01/1987
Copyright © 1987 by American Society for Pharmacology and Experimental Therapeutics






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Copyright © 1987 by the American Society for Pharmacology and Experimental Therapeutics.