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Deficiency in 15-hydroxyprostaglandin dehydrogenase activity after unilateral ureteral obstruction of the dog kidney

PY Wong, JP Pieroni, PH Chao, D Sarubbi, DN Marion, CT Albanase and ED Vaughan

In the present study, metabolism of prostaglandins (PGs) by 15- hydroxyprostaglandin dehydrogenase (15-OH PGDH) was investigated in dog kidneys with ureteral obstruction. After 24 hr of ureteral obstruction, the obstructed kidney and the contralateral kidney were removed and the cytosolic fractions (105,000 X g), enriched in 15-OH PGDH, were prepared from the cortex and medulla. 15-OH PGDH activity was estimated by radiometric assays of the metabolism of [3H]PGE2 and [3H]prostacyclin. Cortical 15-OH PGDH activity decreased by greater than 50% in the ureter-obstructed kidney as compared to the contralateral kidney. Similar results were obtained by estimating the stereo-specific release of tritium from position 15 using 15-[3H]PGF2 alpha as substrate. In contrast to the cortex, there were no differences in 15-OH PGDH activity found in the medulla of the obstructed and contralateral kidneys. Because the cortex contains higher levels of 15-OH PGDH activity, the deficiency in that site may contribute to the elevated levels of PGs in renal venous blood during ureteral obstruction.

Volume 232, Issue 2, pp. 470-474, 02/01/1985
Copyright © 1985 by American Society for Pharmacology and Experimental Therapeutics




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 J. Pharmacol. Exp. Ther.Home page
T. J. Jerde, W. S. Mellon, S. M. Fischer, M. Liebert, D. E. Bjorling, and S. Y. Nakada
Suppression of 15-Hydroxyprostaglandin Dehydrogenase Messenger RNA Concentration, Protein Expression, and Enzymatic Activity during Human Ureteral Obstruction
J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 398 - 403.
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Copyright © 1985 by the American Society for Pharmacology and Experimental Therapeutics.