JPET

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verbeuren, T. J.
Right arrow Articles by Herman, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verbeuren, T. J.
Right arrow Articles by Herman, A. G.

Accumulation and release of [3H]-5-hydroxytryptamine in saphenous veins and cerebral arteries of the dog

TJ Verbeuren, FH Jordaens and AG Herman

Experiments were performed to examine whether isolated canine blood vessels have the ability to accumulate [3H]-5-hydroxytryptamine ([3H]-5- HT) and if so, whether the 3H-amine can be released from the tissues by stimuli known to evoke release of norepinephrine. Helical strips of saphenous veins and cerebral arteries obtained from dogs were incubated with [3H]-5-HT and mounted for superfusion. Both blood vessels take up [3H]-5-HT in a concentration-dependent manner; this accumulation is markedly reduced in veins and arteries denervated with 6- hydroxydopamine and in veins pretreated with cocaine. In saphenous veins and cerebral arteries labeled with [3H]-5-HT, a basal efflux of 3H, consisting mainly of [3H]-5-hydroxyindole-acetic acid, was observed. Electrical stimulation of the preparations evoked a frequency- dependent overflow of tritium due in part to an overflow of intact [3H]- 5-HT. This stimulation-induced 3H-overflow was inhibited by tetrodotoxin and was nearly absent in denervated tissues and in veins pretreated with cocaine. In saphenous veins the overflow of 3H evoked by electrical stimulation was markedly augmented by phentolamine but not influenced by methiothepin. In the veins, both K+ and tyramine caused an overflow of [3H]-5-HT which was absent after denervation or after pretreatment of the preparations with cocaine. Our results show that the major part of the [3H]-5-HT which is taken up by the isolated blood vessels accumulates in the adrenergic nerves and that the indoleamine can be released from these nerves as a "false" transmitter, together with norepinephrine.

Volume 226, Issue 2, pp. 579-588, 08/01/1983
Copyright © 1983 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
I. Cloez-Tayarani and J.-P. Changeux
Nicotine and serotonin in immune regulation and inflammatory processes: a perspective
J. Leukoc. Biol., March 1, 2007; 81(3): 599 - 606.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. L. Cohen, K. W. Schenck, and S. H. Hemrick-Luecke
5-Hydroxytryptamine1A Receptor Activation Enhances Norepinephrine Release from Nerves in the Rabbit Saphenous Vein
J. Pharmacol. Exp. Ther., September 1, 1999; 290(3): 1195 - 1201.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1983 by the American Society for Pharmacology and Experimental Therapeutics.