Effects of d-amphetamine and apomorphine upon operant behavior and schedule-induced licking in rats with 6-hydroxydopamine-induced lesions of the nucleus accumbens

TW Robbins, DC Roberts and GF Koob

After training under a fixed-interval 60 sec schedule of food presentation in the presence of a water tube (to permit schedule- induced licking), groups of rats received either 6-hydroxy-dopamine (6- OHDA)(8 micrograms base/2 microliters) or 0.2% ascorbate-0.9% saline vehicle bilaterally into the nucleus accumbens. 6-OHDA produced greater than 80% depletion of the catecholamines dopamine and norepinephrine and the dopamine metabolite dihydroxyphenylacetic acid in the nucleus accumbens and olfactory tubercle, but nonsignificant depletions in the corpus striatum. The behavior of the groups treated with 6-OHDA ("lesion") and vehicle ("sham") was assessed for up to 58 days postoperatively. In the first few days after 6-OHDA, the lesion group showed reductions in high rates of responding toward the end and in high rates of licking at the beginning of the fixed-interval. However, licking was increased during later portions of the fixed interval in the lesion group. d-Amphetamine (0.25-2.0 mg/kg) increased low rates but decreased high rates of schedule-controlled responding, while generally reducing licking. The lesion group showed attenuated rate- reducing effects of d-amphetamine. In contrast, the lesion group showed enhanced rate-reducing effects of apomorphine (0.025-0.1 mg/kg) on both schedule-controlled responding and schedule-induced licking. In a second determination of the effect of d-amphetamine (0.25-2.0 mg/kg), schedule-induced locomotor activity was recorded and the water tube was removed. The lesion group showed attenuated rate-increasing and rate- decreasing effects of d-amphetamine upon schedule-controlled responding and reductions in the drug-induced increases in locomotor activity. The results are discussed in terms of the functions of dopamine in the control of behavior and in the mediation of the response to d- amphetamine and apomorphine.

Volume 224, Issue 3, pp. 662-673, 03/01/1983
Copyright © 1983 by American Society for Pharmacology and Experimental Therapeutics

This article has been cited by other articles:

				L. H. L. Sellings and P. B. S. Clarke Segregation of Amphetamine Reward and Locomotor Stimulation between Nucleus Accumbens Medial Shell and Core J. Neurosci., July 16, 2003; 23(15): 6295 - 6303. [Abstract] [Full Text] [PDF]

				J. D. Salamone, M. Correa, S. Mingote, and S. M. Weber Nucleus Accumbens Dopamine and the Regulation of Effort in Food-Seeking Behavior: Implications for Studies of Natural Motivation, Psychiatry, and Drug Abuse J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 1 - 8. [Abstract] [Full Text]

				R. Ito, J. W. Dalley, S. R. Howes, T. W. Robbins, and B. J. Everitt Dissociation in Conditioned Dopamine Release in the Nucleus Accumbens Core and Shell in Response to Cocaine Cues and during Cocaine-Seeking Behavior in Rats J. Neurosci., October 1, 2000; 20(19): 7489 - 7495. [Abstract] [Full Text] [PDF]

				D. A. Cory-Slechta, D. J. O'Mara, and B. J. Brockel Nucleus Accumbens Dopaminergic Medication of Fixed Interval Schedule-Controlled Behavior and Its Modulation by Low-Level Lead Exposure J. Pharmacol. Exp. Ther., August 1, 1998; 286(2): 794 - 805. [Abstract] [Full Text]