Histamine receptors in the myenteric plexus-longitudinal muscle of the guinea-pig ileum: H1- and H2-receptor-mediated potentiation of the contractile response to electrical stimulation

JH Zavecz and TO Yellin

The effect of histamine on the contractile response to low frequency- electrical field stimulation in the myenteric plexus-longitudinal muscle preparation of the guinea-pig ileum was investigated. By blocking the direct increase in smooth muscle tone caused by histamine with low concentrations of pyrilamine (10(-9)--5 x 10(-8) M) a dose- dependent, histamine-induced potentiation of the twitch response to electrical stimulation was observed. Blocking the direct actions of histamine with concentrations of pyrilamine greater than 10(-7) M resulted in a biphasic histamine dose-response curve: lower histamine concentrations produced a dose-dependent decrease of the twitch response; higher concentrations produced a potentiation. The potentiating effect of histamine was inhibited by high concentrations of H2-receptor antagonists. Tiotidine (ICI 125, 211) had a pA2 of 5.25, 100 times greater than its pA2 in isolated guinea-pig atria. Blockade of the actions of the H2-receptor agonists dimaprit and tetrahydrozoline also required greater antagonist concentrations. The selective H1-receptor agonist, 2-(2-thiazolyl)-ethylamine, also enhanced the response to electrical stimulation. The potentiating effect of histamine could be blocked by hexamethonium (10(-7)--10(-5) M) but not by atropine. Atropine (10(-9)--10(-8) M) did prevent the decrease in the contraction amplitude induced by histamine in the presence of 10(-7) M pyrilamine. The purinergic antagonist theophylline, adrenergic antagonists or depletion of endogenous catecholamines were without effect. Tetrodotoxin (10(-6) M) inhibited the augmentation induced by histamine. Our results demonstrate that histamine potentiates the acetylcholine-mediated contractile response to electrical field stimulation of guniea-pig ileum via H1- and what may be an H2-receptor subtype.

Volume 223, Issue 1, pp. 177-182, 10/01/1982
Copyright © 1982 by American Society for Pharmacology and Experimental Therapeutics

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