Motor nerve terminal facilitatory action of SQ 20009: an inhibitor of cyclic nucleotide phosphodiesterase

RS Jacobs and DM McNiece

Facilitatory effects of SQ 20009 [1-ethyl-4-(isopropylidenehydrazine)- 1H-pyrazolo-(3,4-b)-pyridine-5-carboxylic acid, ethyl ester, HCl] were investigated at the frog sartorious neuromuscular junction. A dose- dependent increase in miniature end-plate potential frequency occurred without changes in miniature end-plate potential amplitude. Significant increases in the amplitude and the rate of rise of the end-plate potential and reduction in the incidence of end-plate potential failures were observed in magnesium blocked muscle. The increase in end- plate potential amplitude and rate of rise appeared calcium dependent. No significant changes in passive membrane resistance or muscle membrane sensitivity to acetylcholine were observed. Twitch studies employing direct and indirect stimulation of the rat diaphragm preparation demonstrated a preferential facilitation of the indirect response rather than a direct action on the muscle fiber. The facilitatory effect of SQ 20009 on evoked and spontaneous release were of approximately equal orders of magnitude, suggesting that the drug may affect a common mechanism in the two release processes.

Volume 202, Issue 2, pp. 404-410, 08/01/1977
Copyright © 1977 by American Society for Pharmacology and Experimental Therapeutics