JPET Introducing ALZET?ew Model 2006 Pump

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Willson, N. J.
Right arrow Articles by Demartini, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Willson, N. J.
Right arrow Articles by Demartini, J. E.

Effects of methadone hydrochloride on the growth of organotypic cerebellar cultures prepared from methadone-tolerant and control rats

NJ Willson, JF Schneider, L Roizin, JF Fleiss, W Rivers and JE Demartini

Male and female Sprague-Dawley rats were given dl-methadone (5 mg/kg) for at least 3 months and then mated. The drug was continued throughout pregnancy and after delivery. The newly born pups were divided into two groups. One group was tested for in vivo methadone tolerance, while the animals in the othergroup were used to prepare organotypic cerebellar cultures. Various amounts of dl-methadone were added to the media of half of these cerebellum cultures. The effect of the drug in the medium was assessed by measuring explant outgrowth. Similar experiments were carried out with control animals. Statistical analysis of the data obtained in the in vivo portion of the experiment indicates that the pups of methadone-treated mothers tolerate methadone better than those of untreated mothers. The culture experiments revealed that the addition of methadone to the medium reduced explant outgrowth size and this was a dose-related effect. Also, there was significantly less outgrowth from explants prepared using pups of methadone-treated mothers as compared to the controls. There was no significant difference in the effect of methadone on the growth of cultures prepared from the methadone-tolerant and control animals.

Volume 199, Issue 2, pp. 368-374, 11/01/1976
Copyright © 1976 by American Society for Pharmacology and Experimental Therapeutics




This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
P. W. Ackermann, M. Spetea, I. Nylander, K. Ploj, M. Ahmed, and A. Kreicbergs
An Opioid System in Connective Tissue: A Study of Achilles Tendon in the Rat
J. Histochem. Cytochem., November 1, 2001; 49(11): 1387 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
I. Zagon and P. McLaughlin
Increased brain size and cellular content in infant rats treated with an opiate antagonist
Science, September 16, 1983; 221(4616): 1179 - 1180.
[Abstract] [PDF]

Home page
 ScienceHome page
I. Zagon and P. McLaughlin
Naltrexone modulates tumor response in mice with neuroblastoma
Science, August 12, 1983; 221(4611): 671 - 673.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1976 by the American Society for Pharmacology and Experimental Therapeutics.