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Journal of Pharmacology And Experimental Therapeutics, Vol. 187, Issue 2, 332-341, 1973
Copyright © 1973 by American Society for Pharmacology and Experimental Therapeutics

PROPERTIES OF A PURE METABOLICALLY ACTIVE GLOMERULAR PREPARATION FROM RAT KIDNEYS. I. ISOLATION

Elias Meezan 1, Klaus Brendel 1, Judith Ulreich 1, and Edward C. Carlson 1

1 Departments of Pharmacology and Anatomy, University of Arizona Medical Center, Tucson, Arizona

A 99 to 100% pure preparation of rat kidney glomeruli was obtained by a simple procedure involving the perfusion of rat kidneys with magnetic iron oxide suspended in Earle's balanced salt solution buffered with N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid. The glomeruli were isolated with the use of a permanent magnet which allowed tubules and other tissue contaminants which did not contain iron to be removed by decantation. The procedure described was superior to procedures based solely on sieving with regard to the purity of the preparation obtained and its adaptability to different sizes of animals. The iron-containing glomeruli were as active as glomeruli isolated without iron in converting 14C-labeled oleic acid to 14CO2, indicating that the presence of the iron had no deleterious effect on metabolic activity. This would thus appear to be a simple system to study the effect of pharmacologic agents on isolated glomeruli without interference by tubules.

Submitted on February 2, 1973
Accepted on June 7, 1973




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K. Brendel, E. Meezan, and E. C. Carlson
Isolated Brain Microvessels: A Purified, Metabolically Active Preparation from Bovine Cerebral Cortex
Science, September 13, 1974; 185(4155): 953 - 955.
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Copyright © 1973 by the American Society for Pharmacology and Experimental Therapeutics.