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Journal of Pharmacology And Experimental Therapeutics, Vol. 181, Issue 3, 547-554, 1972
Copyright © 1972 by American Society for Pharmacology and Experimental Therapeutics

DETERMINATION OF BARBITURATE DERIVATIVES BY RADIOIMMUANOASSAY

EDWARD J. FLYNN 1 and SYDNEY SPECTOR 1

1 Department of Physiological Chemistry, Pharmocology Section, Roche Institute of Molecular Biology, Nutley, New Jersey

A radioimmunoassay is described in which levels as low as 500 pg of various barbiturates can be detected. The assay method is applicable to the determination of barbiturate levels in plasma, urine on brain homogenates. With this technique, the plasma level of pentobarbital in the rat had a T1/2 of 1.7 hours and barbital a T1/2 of 19.3 hours. The specificity of the antibodies requires a 6-membered ring with an intact urea portion, as well as a cambonyl grouping at positions 4 and 6 on the ring. However, a variety of substitu ents at carbon 5 are allowed without destroying the ability to be recognized by the antibody.

Submitted on January 10, 1972
Accepted on February 16, 1972






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Copyright © 1972 by the American Society for Pharmacology and Experimental Therapeutics.