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1 Departments of Anesthesiology and Pharmacology, College of Medicine, University of Florida, Gainesville, Florida
The original ortho-phthaldialdehyde fluorometric procedure for tissue histamine suffers two drawbacks: 1) interference by other naturally occurring substances, mainly spermidine and histidine, and 2) variability because of the pH sensitivity of the ortho-phthaldialdehydehistamine fluorophor. Both difficulties have been successfully resolved in the present procedure. Under the proper conditions of K2HPO4 and pH, histamine is selectively extracted into isoamyl alcohol, and the fluorescence of the fluorophor is maximized in the presence of citric acid. No interference was obtained with more than 30 other similar and dissimilar compounds. The procedure has been applied to blood and to many tissues from a variety of animals and man. The tissue values for histamine are similar to those detected by bioassay, and the pattern of distribution in the brain of some animals follows that of the other biogenic amines.
Submitted on August 8, 1968
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