JPET

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by SNYDER, S. H.
Right arrow Articles by HENDLEY, E. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by SNYDER, S. H.
Right arrow Articles by HENDLEY, E. D.
Journal of Pharmacology And Experimental Therapeutics, Vol. 164, Issue 1, 90-102, 1968
Copyright © 1968 by American Society for Pharmacology and Experimental Therapeutics

KINETICS OF H3-NOREPINEPHRINE ACCUMULATION INTO SLICES FROM DIFFERENT REGIONS OF THE RAT BRAIN

SOLOMON H. SNYDER 1, ALAN I. GREEN 1, and EDITH D. HENDLEY 1

1 Departments of Pharmacology and Experimental Therapeutics and Psychiatry and the Behavioral Sciences, The Johns Hopkins Univercity School of Medicine, Baltimore, Maryland

The accumulation of H3-norepinephrine into slices from seven different areas of rat brain has been studied. There were marked regional differences in amine accumulation. The striatum showed the greatest accumulation, the cerebellum and medulla oblongatapons had lowest values and the hypothalamus, hippocampus, midbrain and cerebral cortex were intermediate. Amine accumulation was linear in all areas for 45 min. C14-urea was taken up to a similar extent by all regions and equilibrated between slice and medium with 5 min of incubation. There were regional differences in the susceptibility of H3-norepinephrine accumulation to inhibition by desmethylimipramine or reserpine. The striatum and cerebellum were least and most sensitive, respectively, to the effects of these drugs. Desmethylimipramine inhibited amine accumulation with both 5- and 45-min incubations, whereas reserpine was effective only with a 45-min incubation. When brain slices were incubated with concentrations of H3-norepinephrine varying from 0.05 to 0.80 µM, regional differences in saturation of amine accumulation were detected. The cerebellum was the only area in which amine accumulation was not saturable. In the other regions, graphic analysis permitted estimation of Km values. The midbrain and striatum showed the highest Km values, 2.0 x 10-6 M and 1.0 x 10-6 M, respectively; Km values in the other areas were between 4.0 x 10-7 M and 5.0 x 10-7 M. These findings suggest regional differences in neuronal affinity for norepinephrine.

Submitted on March 4, 1968
Accepted on July 27, 1968




This article has been cited by other articles:


Home page
 Arch Gen PsychiatryHome page
S. H. Snyder
Forty Years of Neurotransmitters: A Personal Account
Arch Gen Psychiatry, November 1, 2002; 59(11): 983 - 994.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. G. Phillips and H. C. Fibiger
Dopaminergic and Noradrenergic Substrates of Positive Reinforcement: Differential Effects of d- and l-Amphetamine
Science, February 9, 1973; 179(4073): 575 - 577.
[Abstract] [PDF]

Home page
 ScienceHome page
J. T. Coyle and S. H. Snyder
Antiparkinsonian Drugs: Inhibition of Dopamine Uptake in the Corpus Striatum as a Possible Mechanism of Action
Science, November 14, 1969; 166(3907): 899 - 901.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics.