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Journal of Pharmacology And Experimental Therapeutics, Vol. 159, Issue 2, 255-260, 1968
Copyright © 1968 by American Society for Pharmacology and Experimental Therapeutics

EFFECTS OF HEMICHOLINIUM ON CHOLINE UPTAKE IN THE ISOLATED PERFUSED RABBIT HEART

G. G. Buterbaugh 1 and J. L. Spratt 1

1 Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, Iowa

The influence of hemicholinium (HC-3) on the in vitro uptake of C14-choline by the rabbit heart was studied. The hearts were perfused 1 hr in an oxygenated recycling system at 37°c with eserine-treated Locke-Ringer's solution, containing 0.012 µml of C14-choline and 50 µg/ml of HC-3. Control hearts received no HC-3. Maximum uptake occurred in 60 min. Immediately after perfusion, the hearts were ground in liquid nitrogen and the C14-labeled compounds were quantitatively extracted (control recoveries of 94 ± 3%) and separated by paper chromatography. The results indicated a marked reduction in C14-choline uptake in the presence of HC-3 (62 ng/g vs. 24 ng/g). No incorporation of the C14-label into acetylcholine was observed.

Submitted on April 13, 1967
Accepted on September 27, 1967






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Copyright © 1968 by the American Society for Pharmacology and Experimental Therapeutics.