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Journal of Pharmacology And Experimental Therapeutics, Vol. 157, Issue 3, 503-508, 1967
Copyright © 1967 by American Society for Pharmacology and Experimental Therapeutics

APPLICATION OF ISOTOPE DERIVATIVE TECHNIQUE TO ASSAY OF SECONDARY AMINES: ESTIMATION OF DESIPRAMINE BY ACETYLATION WITH H3-ACETIC ANHYDRIDE

Wolfgang M. Hammer 1 and Bernard B. Brodie 1

1 Laboratory of Chemical Pharmacology, National Heart Institute, National Institutes of Health, Bethesda, Maryland

The isotope derivative technique has been applied to the determination of desipramine (DMI) in plasma. The drug is extracted from alkaline-treated plasma into hexane and acetylated with H3-acetic anhydride to form an amide, which is assayed by scintillation spectrometry. The method is technically simple with a standard deviation of ±4% and a lower limit of sensitivity of 5 mµg/ml of plasma. Preliminary results show that the concentrations of DMI in plasma of subjects receiving the drug in the usual therapeutic doses are in the range of 25 to 100 µg/liter.

Submitted on January 19, 1967
Accepted on March 22, 1967




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Copyright © 1967 by the American Society for Pharmacology and Experimental Therapeutics.