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Journal of Pharmacology And Experimental Therapeutics, Vol. 156, Issue 3, 500-506, 1967
Copyright © 1967 by American Society for Pharmacology and Experimental Therapeutics

A RADIOMETRIC MICROASSAY OF ACETYLCHOLINESTERASE

Lincoln T. Potter 1

1 Department of Pharmacology, Harvard Medical School, Boston, Massachusetts

Several scale modifications are given of a simple radiometric method for determining the activity of acetylcholinesterases. Enzyme samples of 0.01 to 100 µl are used to reconstitute a dried buffer-substrate containing radioactive acetylcholine. Labeled acetate produced by enzymatic hydrolysis of acetylcholine is extracted into an organic solvent and is counted by liquid scintillation spectrometry. The potential of the method is illustrated with two enzyme preparations, highly purified acetylcholinesterase from the electric eel and esterases present in homogenates of the rat brain cortex. Quantities of brain tissue between 0.1 mµg and 3 mg were readily studied.

Submitted on September 30, 1966
Accepted on December 2, 1966




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Copyright © 1967 by the American Society for Pharmacology and Experimental Therapeutics.