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Journal of Pharmacology And Experimental Therapeutics, Vol. 156, Issue 1, 114-125, 1967
Copyright © 1967 by American Society for Pharmacology and Experimental Therapeutics

HISTAMINE IN ISOLATED SMALL NERVE ENDINGS AND SYNAPTIC VESICLES OF RAT BRAIN CORTEX

K. Kataoka 1 and E. De Robertis 1

1 Instituto de Anatomía General y Embriología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina

The subcellular localization of histamine was studied in 24 fractions and subfractions of rat brain cortex. in the homogenate 49 ± 2.8 ng/g of histamine were found by bioassay. The highest percentage and relative specific concentration is in the heavy microsomal fraction, which contains small nerve endings that can be separated at 20,000 x g for 30 mim. The subfractionation of the crude mitochondrial fraction on a density gradient also demonstrated that histamine is in small nerve endings and not in myelin or in free mitochondria. By subfractionation of the heavy microsomes on a gradient, two fractions containing about six times more histamine than in the homogenate were obtained. By electron microscopy small nerve endings were found in them. After osmotic shock of the crude mitochondrial and microsomal-20 fractions a high concentration of histamine is observed in those subfractions containing synaptic vesicles. The presence of histamine in small nerve endings and in synaptic vesicles of the brain cortex suggests a possible synaptic role for this biogenic amine.

Submitted on April 26, 1966
Accepted on September 22, 1966




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Copyright © 1967 by the American Society for Pharmacology and Experimental Therapeutics.