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1 Laboratory of Clinical Science, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland
A sensitive, specific and relatively simple and rapid enzymatic-isotopic method for the estimation of histamine in biologic materials is described. Tissues are incubated with tracer amounts of H3-histamine and C14-S-adenosylmethionine in the presence of the enzyme, histamine methyl transferase, which specifically methylates histamine. H3-C14-methylhistamine istamine is the only radioactive product extracted. The ratio of C14/H3 is directly proportional to the amount of unlabeled tissue histamine present in the incubation mixture. This assay can be utilized to detect as little as 2 ng of tissue histamine.
Submitted on February 25, 1966
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