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Journal of Pharmacology And Experimental Therapeutics, Vol. 151, Issue 1, 46-54, 1966
Copyright © 1966 by American Society for Pharmacology and Experimental Therapeutics

BIOCHEMICAL AND MICROFLUOROMETRIC STUDIES OF NOREPINEPHRINE REDISTRIBUTION ACCOMPANYING SYMPATHETIC NERVE STIMULATION

C. N. Gillis 1, F. H. Schneider 1, L. S. Van Orden 1, and N. J. Giarman 1

1 Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut

Cats were given intravenously 5 µg of norepinephrine-3H per kg 3 hr prior to their use for the preparation of isolated sympathetically innervated atria. In such preparations, sympathetic nerve stimulation at 1 cps for 50 min tended to increase the retention of norepinephrine-3H and significantly decreased the endogenous catecholamine content of the atria. As a result of these changes, the specific activity of norepinephrine-3H in these atria was significantly greater (P < .01) than that of unstimulated control preparations. The specific activity was not significantly greater after stimulation for the same time at 10 or 60 cps. A fluorescence microscope suitably modified for photometry was used for the quantitative determination of fluorescence in the adrenergic nerve fibers of the atria employed in this study. The fluorescent emission maximum of these fibers was identical with that of catecholamine-formaldehyde condensation products. The number and intensity of fluorescent adrenergic fibers of the atria stimulated at 1 cps for 50 min was much greater than those of incubated, unstimulated preparations. The fluorescence of fibers in the stimulated preparations was similar to that of "fresh control" atria (i. e., those not exposed to the incubation medium). The difference between control and stimulated atria was less pronounced when stimulation was carried out for 50 min at 10 cps; and this difference was absent with stimulation at 60 cps. These results are interpreted as supporting the concept of norepinephrine rebinding during low frequency stimulation of adrenergic nerves.

Accepted on August 18, 1965




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G. K. Aghajanian and F. E. Bloom
Electron-Microscopic Autoradiography of Rat Hypothalamus after Intraventricular H3-Norepinephrine
Science, July 15, 1966; 153(3733): 308 - 310.
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Copyright © 1966 by the American Society for Pharmacology and Experimental Therapeutics.