JPET

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stavinoha, W. B.
Right arrow Articles by Ryan, L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stavinoha, W. B.
Right arrow Articles by Ryan, L. C.
Journal of Pharmacology And Experimental Therapeutics, Vol. 150, Issue 2, 231-235, 1965
Copyright © 1965 by American Society for Pharmacology and Experimental Therapeutics

ESTIMATION OF THE ACETYLCHOLINE CONTENT OF RAT BRAIN BY GAS CHROMATOGRAPHY

William B. Stavinoha 1 and Leonard C. Ryan 1

1 Civil Aeromedical Research Institute, Federal Aviation Agency, Pharmacology-Biochemistry Laboratory, Oklahoma City, Oklahoma

A gas chromatographic method has been adapted for the analysis of acetylcholine in rat brain. Rat brains were frozen, extracted in acid ethanol, flash evaporated, taken up in water, reacted with borohydride and the resulting ethanol estimated on a gas chromatograph. The compounds considered possible interfering substances in the bioassay do not interfere with the estimation of acetylcholine by the chemical method. Acetylcholine levels measured by the chemical method and by bioassay are similar.

Accepted on June 17, 1965






Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 1965 by the American Society for Pharmacology and Experimental Therapeutics.