STUDIES OF K⁴² MOVEMENT BETWEEN THE BLOOD AND THE CEREBROSPINAL FLUID OF CATS

¹ Department of Pharmacology, College of Medicine, University of Cincinnati, Cincinnati, Ohio

The movement of K⁴² between blood and the perfused CSF has been studied in anesthetized cats. Premedication with atropine did not affect the net movement of intravenously administered K⁴² from blood to perfusate. Addition of physostigmine or pilocarpine to the premedication decreased the net amount transferred whereas neostigmine had no significant effect. HC-3 increased the net amount of K42 reaching the perfusing CSF. These results implicate a cholinergic receptor in the transfer mechanism.

Pretreatment with acetazolamide, a number of digitalis glycosides and 2, 4-dinitrophenol all decreased the amount of K⁴² which reached the CSF.

When the K⁴² was added in varying concentrations to the perfusing CSF, the concentration in the perfused artificial CSF tended toward the normal value of 2.86 ± 0.26 mEq/l. This method also revealed evidence for an active component of potassium removal from ventricular CSF.

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