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Journal of Pharmacology And Experimental Therapeutics, Vol. 143, Issue 2, 199-204, 1964
Copyright © 1964 by American Society for Pharmacology and Experimental Therapeutics

THE DETERMINATION AND DISTRIBUTION OF POLYAMINES IN MAMMALIAN NERVOUS SYSTEM

Hirotoshi Shimizu 1, Yasuo Kakimoto 1, and Isamu Sano 1

1 Department of Neuropsychiatry, Osaka University Medical School, Osaka, Japan

A method for the determination of spermidine and spermine in nervous tissue is described. The method consists in separation of polyamines from other basic compounds on an ion exchange resin, followed by isolation of spermidine and spermine by ion exchange chromatography and quantification of the amines by reaction with 2,4-dinitrofluorobenzene. The specificity of the method was confirmed by paper chromatography, electrophoresis and by the isolation of spermine and spermidine.

The distribution of spermine and spermidine was examined in the central nervous system and in a peripheral nerve. Completely different distributions were found of these two amines; a higher concentration of spermine was found in the gray matter while spermidine was preponderant in the white matter and peripheral nerve.

Submitted on July 15, 1963
Accepted on October 7, 1963




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Copyright © 1964 by the American Society for Pharmacology and Experimental Therapeutics.