RELEASE OF PHARMACOLOGICALLY ACTIVE SUBSTANCES FROM THE RAT SKIN IN VIVO FOLLOWING THERMAL INJURY

¹ Departamento de Farmacologia, Universidade de São Paulo Faculdade de Medicina de Ribeirão Prêto, São Paulo, Brazil
² Department of Preventive Medicine, University of Illinois College of Medicine, Chicago, Illinois

By using suitable biological assays (guinea pig ileum, rat uterus, hen's rectal cecum, rat's blood pressure, and rat's duodenum) evidence is presented to indicate that histamine, bradykinin, and adenyl compounds (adenosine and/or adenylic acid) appear in the wash fluid from the rat's dorsal subcutaneous air pockets after immersing the outer skin in a water bath at 96°C for 15 seconds. Similar results were obtained when the air pocket was perfused and the skin burned by a 250-watt infrared lamp. There was a good parallelism between the amounts of histamine released under diverse conditions and the amount of bradykinin released. Diffusates into the fluid of the air pockets after burning were freeze-dried and fractionated by dialysis and 80% ethanol precipitation. The ethanol precipitate, which was more or less free of histamine and bradykinin when tested on the rat's blood pressure and the hen's rectal cecum, presented a discrepancy which suggested that the material present differed somewhat from adenosine. No direct examination for serotonin was made but indirect evidence was suggestive but not conclusive.

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