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Journal of Pharmacology And Experimental Therapeutics, Vol. 127, Issue 3, 175-177, 1959
Copyright © 1959 by American Society for Pharmacology and Experimental Therapeutics


A FLUOROMETRIC PROCEDURE FOR THE MEASUREMENT OF TRYPTAMINE IN TISSUES

Sidney M. Hess 1 and Sidney Udenfriend 1

1 Laboratory of Clinical Biochemistry, National Heart Institute, National Institutes of Health, Bethesda, Maryland

A fluorometric method sufficiently sensitive to determine 0.1 to 5 µg of tryptamine in body fluids and tissues is described. The method involves an extraction with benzene, cyclization with formaldehyde to produce tetrahydronorharman followed by a dehydrogenation to form the highly fluorescent product norharman. By omitting the extraction, the procedure can be applied to measure tryptophan in fingertip blood.

Submitted on June 15, 1959




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Copyright © 1959 by the American Society for Pharmacology and Experimental Therapeutics.