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1 Laboratory of Clinical Biochemistry, National Heart Institute, National Institutes of Health, Bethesda, Maryland
A fluorometric method sufficiently sensitive to determine 0.1 to 5 µg of tryptamine in body fluids and tissues is described. The method involves an extraction with benzene, cyclization with formaldehyde to produce tetrahydronorharman followed by a dehydrogenation to form the highly fluorescent product norharman. By omitting the extraction, the procedure can be applied to measure tryptophan in fingertip blood.
Submitted on June 15, 1959
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