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Journal of Pharmacology And Experimental Therapeutics, Vol. 124, Issue 2, 115-119, 1958
Copyright © 1958 by American Society for Pharmacology and Experimental Therapeutics

SUBCELLULAR LOCALIZATION OF RADIOACTIVE DIGITOXIN

James L. Spratt 1 and George T. Okita 1

1 Argonne Cancer Research Hospital, USAEC, and the Department of Pharmacology, The University of Chicago, Chicago, Illinois

The essential experimental findings of this study are listed as follows.

Twenty minutes after a single intravenous injection, the rat essentially clears its vascular system of digitoxin with a significant amount of metabolism of the parent compound.

Upon cell fractionation of in vivo preparations, over 90% of the unchanged digitoxin present in the cell is found in the soluble supernate of rat heart, kidney and liver cells. No marked quantitative binding could be demonstrated with any of the particulate fractions. The results of the in vitro experiments agree with the in vivo work.

Washing of the particulate fractions does lower the binding capacities of these fractions, but does not change the gross distribution picture.

The presence or absence of nonradioactive drug in the suspending media exerts no significant effect on the binding distribution.

Submitted on May 8, 1958






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Copyright © 1958 by the American Society for Pharmacology and Experimental Therapeutics.