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1 Laboratory of Chemical Pharmacology, National Heart Institute, National Institute of Health, Public Health Service, U. S. Department of Health, Education, and Welfare, Bethesda, Maryland
1. A fluorometric method for the determination of serotonin (5-hydroxytryp-tamine) in brain is described. The procedure involves the use of a spectrophoto-fluorometer which can activate and measure emitted fluorescence continuously from 250 to 650 mµ. Serotonin is activated maximally at 295 mµ and emits fluorescent light which shows a maximum at 550 mµ.
2. With this method serotonin has been shown to be a normal constituent of brain.
3. The fluorescent procedure may be applied to the measurement of serotonin in other tissues.
Submitted on December 12, 1955
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