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1 Department of Pharmacology, Northwestern University Medical School, Chicago 11, Illinois
A detailed method for the biological assay of histamine which is an application of established statistical procedures has been described. It is concluded that the method is reliable and valid within the limits of experimental error inherent in the method. The design can easily be adapted to the assay of a large number of histamine solutions in that it is possible to assay eight different unknown solutions concomitantly in a period of about 60 minutes utilizing two isolated tissue baths.
The results of the biological assay of over 300 histamine solutions indicate that the average Standard Error of Estimate of the method is about ± 10 per cent.
Submitted on February 3, 1955
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