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Journal of Pharmacology And Experimental Therapeutics, Vol. 108, Issue 4, 385-392, 1953
Copyright © 1953 by American Society for Pharmacology and Experimental Therapeutics

THE EFFECT OF VASODILATORS UPON THE DEPHOSPHORYLATING ENZYMES OF DOGS' CORONARY ARTERIES

C. Jelleff Carr 1, F. K. Bell 1, Mary F. Bradyhouse 1, and John C. Krantz Jr. 1

1 Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland

1. The optimum hydrogen ion concentration for the in vitro activity of the dephosphorylating enzymes of the coronary arteries of the dog is between a pH of 7.5 and 8.5. There is no difference in Ca++ or Mg++ activation at this pH range.

2. The greatest enzyme activity was obtained with a Ca++ concentration of 3 x 10-3 M or a Mg++ concentration of 1 x 10-2 M.

3. Of thirteen substrates tested only ATP and ADP were acted upon by the enzymes to liberate phosphate.

4. Tissue concentration-activity studies have revealed that the enzyme concentration is related to the quantity of tissue homogenate employed. The tissue concentration-activity relation is linear.

5. In substrate limited systems with ATP the amount of substrate is critical below a concentration of 1.5 x 10-3 M.

6. Vasodilating drugs have not been found to influence these dephosphorylating enzymes in a regular manner. Presumably some other facet of the energy transfer cycle is involved in the mechanism of action of these drugs.

Submitted on February 11, 1953






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Copyright © 1953 by the American Society for Pharmacology and Experimental Therapeutics.