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Journal of Pharmacology And Experimental Therapeutics, Vol. 108, Issue 3, 274-280, 1953
Copyright © 1953 by American Society for Pharmacology and Experimental Therapeutics

IN VITRO EFFECTS OF OUABAIN ON SLICES AND MITOCHONDRIAL PREPARATIONS FROM HEART AND BRAIN

H. Langemann 1, T. M. Brody 2, and J. A. Bain 3

1 Pharmacological Institute, University of Zurich, Zurich, Switzerland
2 Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan
3 Departments of Pharmacology and Psychiatry, College of Medicine, University of Illinois, Chicago 12, Illinois

Ouabain in concentrations of from 10-6 to 10-3 M produced a sustained increase in the respiratory rate of cat heart slices. Such a stimulation could only be demonstrated after depression of the original respiratory rate by preincubation or by treatment with barbiturate. When the glycoside was added to undepressed slices the respiration was inhibited by all concentrations of ouabain from 10-6 to 10-3 M.

Ouabain had no effect upon the oxidative or phosphorylative capacity of cat heart or guinea pig brain mitochondrial preparations, nor did the drug stimulate such mitochondrial preparations which had been depressed by barbiturate or calcium. The results are interpreted as indicating that the effects of ouabain on intact heart cells are mediated by some other means than a direct action upon oxidative enzymes per se.

Submitted on January 23, 1953






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